Use of a Facial Moisturizer Containing Pal-KTTKS Peptide
Rosemarie Osborne, Ph.D.; Larry Robinson, Ph.D.; Lisa Mullins, B.S.; Patricia Raleigh, B.S.; James Li, M.S., M.A.; Kukizo Miyamoto, Ph.D.;
Procter & Gamble Miami Valley Innovation Center, Beauty Technology Division
Pal-KTTKS is a pentapeptide that, as a fragment of collagen, acts in vitro to stimulate synthesis of connective tissue composed of proteins (collagen I, III, IV) and polysaccharides (GAGS, hyaluronic acid), as part of a feedback mechanism in fibroblasts. Previous clinical studies have demonstrated that use of facial moisturizers containing pal- KTTKS can lead to improvements in the appearance of photoaged skin. In the present study, the facial benefits of a pal-KTTKScontaining moisturizer on the appearance of facial texture, fine lines and wrinkles were determined.
Pal-KTTKS (palmitoyl-lys-thr-thr-lys-ser) was obtained from Sederma, France. A unique aminopeptide containing formulation was evaluated, which also contained niacinamide, panthenol, and vitamin E in a serum-type facial moisturizer product at a concentration of 3 ppm. Responses were compared to a moisturizer base vehicle.
Clinical Facial Study Protocol
The clinical study was a double-blinded 8-week facial study on 35 to 65 year old women. The women applied the moisturizer products twice daily in a split face, randomized round robin study design. The study was designed so that there were 60 panelists per product. At baseline, 4 and 8 weeks, digital facial images were obtained and analyzed for changes in facial texture, fine lines and wrinkles by expert and self-grading, and by image analysis.
To evaluate the facial benefits of a moisturizer containing pal-KTTKS on the appearance of bumpy texture, fine lines and wrinkles in women with photoaged skin.
(Insert two bar graphs and skin image)
The pal-KTTKS product improved the appearance of bumpy facial texture (Fig. 1) and fine
lines/wrinkles (Fig. 2), as determined by imaging with quantitative analysis or expert
Improvements in facial skin texture, fine lines and wrinkles are apparent in the under-eye and cheek areas of the face
These results demonstrate the benefits of a facial moisturizer containing Pal-KTTKS for
improving the appearance of facial skin aging.